School of Medicine - Basic Medical ScienceBiochemistry I

JapaneseEnglish

Website

生化学1集合

DNA double-strand break (DSB) is one of the most serious threats for cells. Improper repair of DSBs leads to cell death or carcinogenesis. DSBs can be induced by exogenous stimuli such as ionizing radiation, and endogenous normal cellular processes such as DNA replication, meiosis and V(D)J recombination. Since we found 53BP1 as a tumor suppressor p53-binding protein in 1994, many laboratories, including ours, have shown that 53BP1 is involved in cellular responses to DSBs.
Goals in our research are:
1) clarifying molecular mechanisms for the cell-cycle checkpoints and repair of DSBs.
2) generating a novel method in radiation therapy for cancer stem cells.

Contact Information

TEL: 076-218-8109 / FAX: 076-286-4693 / Email: biochem1@kanazawa-med.ac.jp

Faculty

Professor and Chair

  • IWABUCHI Kuniyoshi 生化学Ⅰ-3岩淵

Senior Assistant Professor

  • SUNATANI Yumi
  • SAKASAI Ryo

Assistant Professor

  • MATSUI Tadashi

Research Assistant

  • YAMANADA Eiko

Secretary

  • TANIHARA Kiyomi

Research Achievements

Research Activities

  • Okamoto Y, Iwasaki WM, Kugou K, Takahashi KK, Oda A, Sato K, Kobayashi W, Kawai H, Sakasai R, Takaori-Kondo A, Yamamoto T, Kanemaki MT, Taoka M, Isobe T, Kurumizaka H, Innan H, Ohta K, Ishiai M, TakataM. Replication stress induces accumulation of FANCD2 at central region of large fragile genes. Nucleic Acids Res. 2018, 46(6): 2932-2944, DOI: 10.1093/nar/gky058
  • Sunatani Y, Kamder RP, Sharma MK, Matsui T, Sakasai R, Hashimoto M, Ishigaki Y, Matsumoto Y, Iwabuchi K. Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase. Exp. Cell Res. 2018, 362(2): 450-460, doi: 10.1016/j.yexcr.2017.12.009.
  • Sakasai R, Isono M, Wakasugi M, Hashimoto M, Sunatani Y, Matsui T, Shibata A, Matsunaga T, Iwabuchi K. Aquarius is required for proper CtIP expression and homologous recombination repair. Sci. Rep. 2017, 7(1):13808, doi: 10.1038/s41598-017-13695-4
  • Matsui Y, Sunatani Y, Hayashi N, Okino K, Okushi Y, Mukai K, Adachi H, Yamaya H, Iwabuchi K, Yokoyama H. DNA double-strand breaks induced intractable glomerular fibrosis in renal allografts.
    Clin Exp Nephrol. 2016, 20 (3): 479-488, doi: 10. 1007/s10157-015-1174-3.
  • Iimori M, Watanabe S, Kiyonari S, Matsuoka K, Sakasai R, Saeki H, Oki E, Kitao H, Maehara Y. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome instability.
    Nat Commun. 2016, doi: 10.1038/ncomms11117.

External Research Funding

  • Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (18H03375). 2018-2020, Iwabuchi K.
  • Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (18K11648). 2018-2020, Sakasai R.
  • Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15K12210). 2015-2017, Iwabuchi K.
  • Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15K00546). 2015-2017, Matsui T.
  • Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15K16127). 2015-2017, Sakasai R.